recombinant angptl4 peptides  (R&D Systems)

Journal: Oncology reports

Article Title: Expression of angiopoietin-like 4 (ANGPTL4) in human colorectal cancer: ANGPTL4 promotes venous invasion and distant metastasis.

doi: 10.3892/or.2011.1176

Figure Lengend Snippet: Figure 1. Immunohistochemical staining for ANGPTL4 in human tissues. (A) Faintly expression in the surface lining cells of normal colon mucosa (arrow- heads), (B) strongly in cytoplasm of moderately-differentiated adenocarcinoma, and (C) poorly-differentiated adenocarcinoma (non-solid type) of human colorectal cancer (magnification, x100).

Article Snippet: Deparaffinized sections were preincubated with normal rabbit serum to prevent non-specific binding, and then incubated overnight at 4 ̊C with an optimal dilution (0.1 μg/ml) of a primary polyclonal goat antibody against human Angptl4 (r&D systems, Inc., minneapolis, mA, usA). the slides were sequentially incubated with a biotinylated rabbit antigoat immunoglobulin antibody, and the reaction products were viewed using diaminobenzidine (DAb; Dako ltd.) and counterstained with hematoxylin. primary antibody preabsorbed with excess recombinant Angptl4 peptides (r&D systems, Inc.) was used as negative control.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Oncology reports

Article Title: Expression of angiopoietin-like 4 (ANGPTL4) in human colorectal cancer: ANGPTL4 promotes venous invasion and distant metastasis.

doi: 10.3892/or.2011.1176

Figure Lengend Snippet: Figure 2. Overall survival based on the expression of ANGPTL4 in human colorectal cancer (60 cases). ANGPTL4 expression was not associated with the overall survival in univariate survival analysis. (p=0.455 in log-rank test).

Article Snippet: Deparaffinized sections were preincubated with normal rabbit serum to prevent non-specific binding, and then incubated overnight at 4 ̊C with an optimal dilution (0.1 μg/ml) of a primary polyclonal goat antibody against human Angptl4 (r&D systems, Inc., minneapolis, mA, usA). the slides were sequentially incubated with a biotinylated rabbit antigoat immunoglobulin antibody, and the reaction products were viewed using diaminobenzidine (DAb; Dako ltd.) and counterstained with hematoxylin. primary antibody preabsorbed with excess recombinant Angptl4 peptides (r&D systems, Inc.) was used as negative control.

Techniques: Expressing

Journal: Oncology reports

Article Title: Expression of angiopoietin-like 4 (ANGPTL4) in human colorectal cancer: ANGPTL4 promotes venous invasion and distant metastasis.

doi: 10.3892/or.2011.1176

Figure Lengend Snippet: Figure 3. RT-PCR and Western blot analysis for ANGPTL4 in human colo rectal cancer: tissues and cultured cell lines. (A) RT-PCR. M, 100 bp ladder marker (Invitrogen, Inc.). (B) Western blot analysis. Human colorectal cancer tissues (1-6) and human colorectal cancer cell lines (7-12). [1, Cancer tissue; cases 1 and 2, normal gastric tissue; cases 1 and 3, cancer tissue; cases 2 and 4, normal gastric tissue; cases 2, 5, cancer tissue; cases 3 and 6, normal gastric tissue; cases-3 and 7, Caco-2; 8, Colo201; 9, Colo320DM; 10, DLD-1; 11, WiDr,; 12, LS123].

Article Snippet: Deparaffinized sections were preincubated with normal rabbit serum to prevent non-specific binding, and then incubated overnight at 4 ̊C with an optimal dilution (0.1 μg/ml) of a primary polyclonal goat antibody against human Angptl4 (r&D systems, Inc., minneapolis, mA, usA). the slides were sequentially incubated with a biotinylated rabbit antigoat immunoglobulin antibody, and the reaction products were viewed using diaminobenzidine (DAb; Dako ltd.) and counterstained with hematoxylin. primary antibody preabsorbed with excess recombinant Angptl4 peptides (r&D systems, Inc.) was used as negative control.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Cell Culture, Marker